MersV1, Main, Exploration, bibRecord, 003992

Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis.

Identifieur interne : 003992 ( Main/Exploration ); précédent : 003991; suivant : 003993

Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis.

Auteurs : J G Duman [États-Unis] ; N. Li ; D. Verleye ; F W Goetz ; D W Wu ; C A Andorfer ; T. Benjamin ; D C Parmelee

Source :

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology [ 0174-1578 ] ; 1998.

RBID : pubmed:9591363

Descripteurs français

KwdFr :
- ADN complémentaire (génétique), Animaux, Congélation, Données de séquences moléculaires, Glycoprotéines (génétique), Larve (génétique), Poissons, Protéines antigel, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique.
MESH :
- génétique : ADN complémentaire, Glycoprotéines, Larve.
- Animaux, Congélation, Données de séquences moléculaires, Poissons, Protéines antigel, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antifreeze Proteins, Base Sequence, Coleoptera (genetics), Coleoptera (growth & development), DNA, Complementary (genetics), Fishes, Freezing, Glycoproteins (genetics), Larva (genetics), Molecular Sequence Data, Sequence Homology, Amino Acid.
MESH :
- chemical , genetics : DNA, Complementary, Glycoproteins.
- chemical : Antifreeze Proteins.
- genetics : Coleoptera, Larva.
- growth & development : Coleoptera.
- Amino Acid Sequence, Animals, Base Sequence, Fishes, Freezing, Molecular Sequence Data, Sequence Homology, Amino Acid.

Abstract

The deduced amino acid sequences of antifreeze proteins (AFPs) from larvae of the beetle Dendroides canadensis were determined from both complementary DNAs (cDNAs) and from peptide sequencing. These consisted of proteins with a 25-residue signal peptide and mature proteins 83 (Dendroides antifreeze protein; DAFP-1) or 84 (DAFP-2) amino acids in length which differed at only two positions. Peptide sequencing yielded sequences which overlapped exactly with those of the deduced cDNA sequences of DAFP-1 and DAFP-2, while the partial sequence of another AFP (DAFP-3) matched 21 of 28 residues. Seven 12- or 13-mer repeating units are present in these antifreeze proteins with a consensus sequence consisting of: Cys-Thr-X3-Ser-X5-X6-Cys-X8-X9-Ala-X11-Thr-X1 3, where X3 and X11 tend toward charged residues, X5 tends toward threonine or serine, X6 toward asparagine or aspartate, X9 toward asparagine or lysine, and X13 toward alanine in the 13-mers. The most interesting feature of these proteins is that throughout the length of the mature antifreeze proteins every sixth residue is a cysteine. These sequences are not similar to any of the known fish AFPs, but they are similar to AFPs from the beetle Tenebrio molitor.

DOI: 10.1007/s003600050140
PubMed: 9591363

Affiliations:

Le document en format XML

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<term>Coleoptera (genetics)</term>
<term>Coleoptera (growth & development)</term>
<term>DNA, Complementary (genetics)</term>
<term>Fishes</term>
<term>Freezing</term>
<term>Glycoproteins (genetics)</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">The deduced amino acid sequences of antifreeze proteins (AFPs) from larvae of the beetle Dendroides canadensis were determined from both complementary DNAs (cDNAs) and from peptide sequencing. These consisted of proteins with a 25-residue signal peptide and mature proteins 83 (Dendroides antifreeze protein; DAFP-1) or 84 (DAFP-2) amino acids in length which differed at only two positions. Peptide sequencing yielded sequences which overlapped exactly with those of the deduced cDNA sequences of DAFP-1 and DAFP-2, while the partial sequence of another AFP (DAFP-3) matched 21 of 28 residues. Seven 12- or 13-mer repeating units are present in these antifreeze proteins with a consensus sequence consisting of: Cys-Thr-X3-Ser-X5-X6-Cys-X8-X9-Ala-X11-Thr-X1 3, where X3 and X11 tend toward charged residues, X5 tends toward threonine or serine, X6 toward asparagine or aspartate, X9 toward asparagine or lysine, and X13 toward alanine in the 13-mers. The most interesting feature of these proteins is that throughout the length of the mature antifreeze proteins every sixth residue is a cysteine. These sequences are not similar to any of the known fish AFPs, but they are similar to AFPs from the beetle Tenebrio molitor.</div>
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Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis.

Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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